Chemical Reagent
Description
Trypsin is a member of the serine protease family. Trypsin will cleave peptides on the C-terminal side of lysine and arginine amino acid residues. The pH optimum of trypsin is pH7 - 10. The enzyme is inhibited by serine proteases inhibitors, e.g. PMSF, and metal
chelating agents, e.g., EDTA. Recombinant Porcine Trypsin is a genetically engineered protein, expressed in E.coli and purified by high pressure liquid chromatography. There is no foreign enzyme activity such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF contain in preparation.
ADVANTAGES
Animal origin free
Recombinant Porcine Trypsin eliminates the risk of viruses, and other potential adventitious agents found in animal derived trypsin. Which belong to the AOF level 3 products.
Stable
A sterile recombinant trpsin lyophilized powder eliminates the contamination opportunity and
decreases the chance of activity loss in the process of transport and storage.
High purity
1)Recombinant porcine trypsin provides increased specificity and eliminates contaminating activities found in lower purity enzymes.
2) No other proteases contaminated such as chymotrypsin, carboxypeptidase A.
3) One single main band at 24kD by SDS-PAGE.
SALIENT FEATURES
Source E. Coli
Purified by HPLC
Physical form White lyophilized powder
Additives Carbohydrates
Protein content 35% ～ 50%
Special activity >3000 USP units/mg pro.
Purity > 95%
Foreign activity No chymotrypsin, carboxypeptidase A, and other protease contaminant.
Product Cat. No. Special activity Packaging Manufacturer
Recombinant Procine Trypsin 1 RPT02040 ≥4000 USP /mg pro 5mg bulkYaxinBio
RPT02030 ≥3000 USP /mg pro 10mg bulk YaxinBio
RPT02025 ≥2500 USP /mg pro 1g bulk YaxinBio
UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute
in a reaction volume of 3.0ml at pH7.6 at 25℃, with BAEE as a substrate
(1cm light path).
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Shanghai Yaxin Biotechnology Co. Ltd.
scientific and technological innovation
Address: Room 101, Building 1, No. 466 Yin Du Road, Xu Hui District, Shanghai, China,,
xuhui, Shanghai
China, 201108
Tel: 86-21-54336592
Fax: 86-21-54336593
