New-type Chemical Materials
Description
for Enzymatic Hydrolysis of Protein,CAS:9002-07-7,Protein sequencing
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Bulk Recombinant Trypsin For Manufacturing Recombinant Insulin
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DESCRIPTION
Recombinant Trypsin
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The pH optimum of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as from carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
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ADVANTAGES
Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.
Stable:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1. Recombinant porcine trypsin provides increased specificity and eliminates contaminating activities found in enzymes with lower purity level.
2. No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
3. One single main band at 24kD by SDS-PAGE.
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MAIN?FEATURES?
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Source | E.?Coli |
Purified?by | HPLC |
Physical?form | White?lyophilized?powder |
Additives | Carbohydrates |
Protein?content | 35%?～?70% |
Specific?activity | ≥3800?USP?units/mg?pro |
Purity | >?99% |
Contaminant?activity | No?chymotrypsin,?carboxypeptidase?A,?and?other?protease?contaminant. |
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PRODUCT?INFORMATION
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UNIT?DEFINITION:One?USP?unit?of?trypsin?activity?will?produce?a?Delta?A253?of?0.003?per?minute?in?a?
???????????????????????????? reaction?volume?of?3.0ml?at?pH7.6?and?25℃,?with?BAEE?as?a?substrate?(1cm?light?path).
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Product | Cat.?No. | Specific?activity | Packaging | Manufacturer |
Recombinant?Porcine?Trypsin | RPT0201 | ≥3800?USP?u/mg?pro | 10mg,100mg,1g?or?bulk | YaxinBio |
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RELATED?PRODUCT
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Recombinant?Carboxypeptidase?B
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Glutamic acid proteases for Peptide fingerprinting analysis / Staphylococcus Aureus v8 Protease...
Shanghai Yaxin Biotechnology Co.,Ltd.
Recombinant Trypsin,Sequencing Grade Trypsin,Recombinant Carboxypeptidase B
Address: No.466 YinDu Road,XuHui, Shanghai, China,
Shanghai, Shanghai
China, 200000
Tel: 86-021-54336592
Fax: 86-021-54336593
