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Carboxypeptidase B Producing recombinant Polypeptide , C-terminal lysine EC 3.4.17.2
RCPB01
Description
producing recombinant Polypeptide,C-terminal lysine EC 3.4.17.2
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Carboxypeptidase B / Recombinant Carboxypeptidase B / RCPB / rCPB
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DESCRIPTION
Staphylococcus Aureus v8 Protease
Carboxypeptidase?B?catalyzes?hydrolysis?of?the?basic?amino?acids?lysine,?arginine?and?histidine?from?the?
C-terminal?end?of?polypeptides.?The?molecular?weight?is?34,500?daltons,?the?pH?optimum?is?8.0,?and?pI?is?6.0
Carboxypeptidase?B?is?competitively?inhibited?by?arginine?and?lysine.?The?enzyme?is?also?inhibited?by?metal?
chelating?agents,?e.g.,?EDTA.?Recombinant?Carboxypeptidase?B?(EC?3.4.17.2)?is?expressed?in?E.Coli?and?
purified?by?high?pressure?liquid?chromatography.?There?is?no?trace?of?other?enzyme?(such?as?
carboxypeptidase?A?and?chymotrypsin)?activity.?No?protease?inhibitors?such?as?PMSF?are?present?in?the?
preparation.
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MAIN?FEATURES
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Source | E.?Coli |
Purified?by | HPLC? |
Physical?form | White?or?white-like?lyophilized?powder |
Additives | Tris?salts,?NaCl?salts?and?carbohydrates |
Protein?content | 35%?～?50% |
Specific?activity | ≥120?units/mg?pro.;?≥170?units/mg?pro |
Purity | >?95% |
Contaminant?activity | No?chymotrypsin,carboxypeptidase?A,and?other?protease?contaminant. Less?than?10?ppm?of?recombinant?trypsin. |
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Shanghai Yaxin Biotechnology Co.,Ltd.
Recombinant Trypsin,Sequencing Grade Trypsin,Recombinant Carboxypeptidase B
Address: No.466 YinDu Road,XuHui, Shanghai, China,
Shanghai, Shanghai
China, 200000
Tel: 86-021-54336592
Fax: 86-021-54336593
