Coloration Products
Description
Carboxypeptidase B for Mass Spectrometry Recombinant Enzyme 170u / mg Pro
?
?
Description
?
Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 33.8 kda, optimun pH-7.9,
and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited
by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA,1,10-phenanthroline.
Synonyms: Carboxypeptidase B, Cpb1, Cpb.
?
Advantages
?
Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or any other potential adventitious agents found in animal-derived carboxypeptitase B
Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.
High purity:
1) Recombinant carboxypeptidase B provides increased specific?activity?and?eliminates contaminating proteases activities found in extracted enzymes with lower purity?level.
2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.
3)Less than 10ppm of recombinant trypsin.
?
?
Main Features
?
- - - -
Source | E. Coli |
Protein content | 35% ～ 60% |
Purity | Single main band on SDS-PAGE |
M.W. | 33.8kD |
Contaminant activity | Less than 10 ppm of recombinant trypsin. |
- - - -
?
?
?
?
?
?
?
Unit Definition
?
One Unit of carboxypeptidase B activity hydrolyzes one micromole of?hippuryl-L-arginine per minute at 25℃, pH 7.65.
?
Recommend Usage
?
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH?7-9.
?
Storage Instructions
?
Recommend recombinant carboxypeptidase B lyophilized?should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90%?activity remained?after 10 times repeated freezing and thawing.
Read More
Shanghai Yaxin Biotechnology Company
Recombinant Carboxypeptidase B,Human Trypsin,Recombinant Enterokinase
Address: No.466 YinDu Road,XuHui, Shanghai, China,
Shanghai, Shanghai
China, 20000
Tel: 86-21-54336592
Fax: 86-21-54336593
