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Glutamic Acid Proteases for Peptide Fingerprinting Analysis Animal Origin Free Enzymes

V813

Description

Glutamic Acid Proteases for Peptide Fingerprinting Analysis ? Animal Origin Free Enzymes

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Description

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Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues.The Optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors are Phosphoric acid diisopropyl ester fluoride (DFP) and alpha 2 - macroglobulin and Na-p-tosyl-L-lysine chloromethyl ketone (TLCK).

CAS:66676-43-5

EC:3.4.21.19

Full Name:Endoproteinase Glu-C,from Staphylococcus aureus strain V8,V8 Protease

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Specific Actiity

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Approx.20U/mg ar 25℃ with Z-Phe-Leu-Glu-4-nitranilide as the substrate(approx.500U/mg at 37℃ with casein as the substrate).

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Recommend Usage

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Cutting condition:PH 8.0~8.5

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Packaging



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1U/vial,50μg/vial

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Storage Instructions

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V8 Protease?should be stored under -20℃ in sealed container.

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Application

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Protein structure and sequence analysis

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Recombinant Carboxypeptidase B,Human Trypsin,Recombinant Enterokinase

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